Homepage of the Lentiviral Gene Ontology Vectors


{Production of viral particles}


{Sample requests}
The links in this section lead to external web pages. I do not have any influence on these web pages.

  • Most LeGO vectors are available through Addgene (more to come):
    http://www.addgene.org/Boris_Fehse
  • Packaging plasmids
    We routinely use 3rd generation packaging (pMDLg/pRRE + pRSV-Rev), but LeGO vectors are compatible with any 2nd or 3rd generation HIV-1 derived packaging system. For example, I have tested psPAX2 2nd generation packaging with very comparable results. You can use the packaging system that you already have in your lab, if you want to. Packaging plasmids are available through Addgene as well:
    http://www.addgene.org/lentiviral/packaging/

    • pMDLg/pRRE
    • pRSV-Rev
    • pMD2.G (Note: this VSV-G plasmid is slightly different from the one in our lab, but it works fine.)


{Other interesting websites}
The links in this section lead to external web pages. I do not have any influence on these web pages.



  © 2015 · Kristoffer Riecken · contact