{RGB-Marking}
Based on LeGO vectors we have developed a new method for color guided clonal cell tracking. By expressing three fluorescent proteins
of the three basic colors red, green and blue at highly different but for each single cell constant levels, almost every color is generated
and allows clonal tracking.
The same principle of color mixing for example is used in computer or TV screens to generate all colors just by mixing the three basic colors.
The publication of this method is available at:
The table below provides information of the three vectors used for RGB-Marking. Maps are available as pdf-files, sequences are in
FASTA-format (text files). There are also map+sequence-files in the pDRAW32 format.
pDRAW32 is a great piece of software, it can do all the virtual cloning,
restriction analysis, primer annealing, virtual PCR and agarose gels, map drawing, primer database and everything else - and its free
(but windows PCs only).
