Lentiviral Gene Ontology Vectors

Homepage of the Lentiviral Gene Ontology Vectors

{New e-mail address}
Please note that I changed my name because of my marriage from Kristoffer Weber to Kristoffer Riecken. My new e-mail address is available on the contact page.

{General information}
This is a list of vectors published in:

- Gomez-Nicola et al., Scientific Reports 4 (2014), Article number: 7520
   http://www.nature.com/srep/2014/141222/srep07520/full/srep07520.html
- Weber et al., Nature Protocols (2012), 7: 839-849
   http://www.nature.com/nprot/journal/v7/n5/abs/nprot.2012.026.html
- Weber et al., Nature Medicine (2011), 17: 504-509
   http://www.nature.com/nm/journal/v17/n4/abs/nm.2338.html
- Weber et al., Gene Therapy (2010), 17(4): 511-520
   http://www.nature.com/gt/journal/v17/n4/abs/gt2009149a.html
- Weber et al., Molecular Therapy (2008), 16(4): 698-706
   http://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(16)31451-4

Maps are available as pdf-files, sequences are in FASTA-format (text files). There are also map+sequence-files in the pDRAW32 format. pDRAW32 is a great piece of software, it can do all the virtual cloning, restriction analysis, primer annealing, virtual PCR and agarose gels, map drawing, primer database and everything else - and its free (runs on Windows or on MacOS/Linux with free emulators).

Many LeGO vectors are available through Addgene.org, see Protocols section.

{1. Vectors for cell marking}

Vector Marker Map Sequence Map+Sequence*

LeGO-Cer2 Cerulean PDF FASTA pDRAW32

LeGO-G2 eGFP PDF FASTA pDRAW32

LeGO-E2 Emerald (EmGFP) PDF FASTA pDRAW32

LeGO-V2 Venus PDF FASTA pDRAW32

LeGO-T2 tdTomato PDF FASTA pDRAW32

LeGO-C2 mCherry PDF FASTA pDRAW32

*use right-klick and "save as" to download file

{3. Vectors for cDNA expression}

Vector Marker Map Sequence Map+Sequence*

LeGO-iCer2 Cerulean PDF FASTA pDRAW32

LeGO-iG2 eGFP PDF FASTA pDRAW32

LeGO-iV2 Venus PDF FASTA pDRAW32

LeGO-iT2 tdTomato PDF FASTA pDRAW32

LeGO-iC2 mCherry PDF FASTA pDRAW32

*use right-klick and "save as" to download file

{4. Vectors for cDNA and shRNA expression}

Vector Marker Map Sequence Map+Sequence*

LeGO-iCer Cerulean PDF FASTA pDRAW32

LeGO-iG eGFP PDF FASTA pDRAW32

LeGO-iE Emerald (EmGFP) PDF FASTA pDRAW32

LeGO-iV Venus PDF FASTA pDRAW32

LeGO-iT tdTomato PDF FASTA pDRAW32

LeGO-iC mCherry PDF FASTA pDRAW32

*use right-klick and "save as" to download file